Table 2.
Effect of RNAi with different silencer constructs containing different promoters or structures of shRNAs in different cell lines.
| Cell types | ||||||||
|---|---|---|---|---|---|---|---|---|
| HEK293 | COS7 | NIH3T3 | HeLa | |||||
| Silencer constructs | G/R ratio | n | G/R ratio | n | G/R ratio | n | G/R ratio | n |
| pSiEnU6-Ctrl | 100.0 ± 4.2 | 175 | 100.0 ± 5.7 | 132 | 100.0 ± 4.9 | 50 | 100.0 ± 2.4 | 207 |
| pSiEnU6-shGFP-1 | 24.9 ± 1.6 | 155 | 17.4 ± 1.5 | 112 | 24.8 ± 1.7 | 115 | 33.2 ± 1.6 | 127 |
| pSiEnU6-shGFP-2 | 12.8 ± 0.5 | 245 | 17.6 ± 1.5 | 135 | 8.9 ± 0.5 | 137 | 20.1 ± 1.1 | 90 |
| pSiEnU6-shGFP-3 | 16.0 ± 0.5 | 369 | 14.1 ± 0.9 | 100 | 13.2 ± 1.4 | 101 | 23.6 ± 1.7 | 88 |
| pSiEnU6-Ctrl CMV-Ago1 | 100.0 ± 5.6 | 125 | 100.0 ± 7.2 | 83 | 100.0 ± 7.8 | 52 | 100.0 ± 6.9 | 95 |
| pSiEnU6-shGFP-1 CMV-Ago1 | 53.3 ± 4.5 | 50 | 63.5 ± 3.3 | 119 | 65.9 ± 4.0 | 87 | 59.7 ± 3.6 | 93 |
| pSiEnU6-shGFP-2 CMV-Ago1 | 25.5 ± 1.6 | 119 | 19.6 ± 1.0 | 108 | 49.0 ± 4.3 | 63 | 32.8 ± 2.0 | 101 |
| pSiEnU6-shGFP-3 CMV-Ago1 | 38.7 ± 2.0 | 102 | 24.4 ± 2.9 | 61 | 56.9 ± 3.9 | 72 | 53.1 ± 3.5 | 135 |
| pSiEnU6-Ctrl CMV-Ago2 | 100.0 ± 4.1 | 195 | 100.0 ± 5.4 | 136 | 100.0 ± 5.9 | 76 | 100.0 ± 3.1 | 224 |
| pSiEnU6-shGFP-1 CMV-Ago2 | 10.6 ± 0.7 | 291 | 9.3 ± 1.0 | 62 | 20.0 ± 2.0 | 44 | 22.6 ± 1.6 | 104 |
| pSiEnU6-shGFP-2 CMV-Ago2 | 5.4 ± 0.5 | 221 | 7.0 ± 0.4 | 121 | 11.5 ± 0.7 | 69 | 13.7 ± 0.8 | 108 |
| pSiEnU6-shGFP-3 CMV-Ago2 | 7.5 ± 0.5 | 90 | 8.0 ± 0.5 | 123 | 13.4 ± 0.9 | 81 | 11.6 ± 0.8 | 87 |
| pSiU6-Ctrl | 100.0 ± 4.3 | 117 | 100.0 ± 3.3 | 146 | 100.0 ± 4.5 | 63 | 100.0 ± 3.0 | 97 |
| pSiU6-shGFP-1mir30 | 7.5 ± 0.5 | 196 | 4.4 ± 0.4 | 37 | 10.6 ± 1.4 | 93 | 21.5 ± 2.1 | 101 |
| pSiU6-shGFP-2mir30 | 7.8 ± 0.5 | 316 | 4.6 ± 0.4 | 75 | 10.9 ± 0.8 | 162 | 24.7 ± 1.7 | 149 |
| pSiU6-shGFP-3mir30 | 32.0 ± 2.7 | 274 | 32.8 ± 2.0 | 54 | 61.1 ± 3.3 | 89 | 40.9 ± 2.2 | 219 |
| pSiU6-Ctrl CMV-Ago2 | 100.0 ± 2.8 | 266 | 100.0 ± 4.0 | 162 | 100.0 ± 3.8 | 116 | 100.0 ± 3.6 | 173 |
| pSiU6-shGFP-1mir30 CMV-Ago2 | 7.8 ± 0.5 | 214 | 13.0 ± 1.3 | 136 | 34.8 ± 4.9 | 76 | 22.3 ± 2.7 | 153 |
| pSiU6-shGFP-2mir30 CMV-Ago2 | 13.2 ± 1.1 | 287 | 15.3 ± 1.4 | 104 | 41.7 ± 4.7 | 105 | 22.2 ± 1.9 | 125 |
| pSiU6-shGFP-3mir30 CMV-Ago2 | 5.0 ± 0.3 | 290 | 9.1 ± 1.6 | 122 | 25.2 ± 3.5 | 97 | 17.1 ± 2.6 | 130 |
Note: Data for pSiEnU6-shGFPs, pSiEnU6-shGFPs CMV-Ago1 and pSiEnU6-shGFPs CMV-Ago2 are from an experiment independent from the data plotted in Figure 2 and Table 1. G/R ratio: green to red fluorescence intensity ratio normalized to controls, which contain no shGFP or shGFPmir30 expression in the same pSi configuration. n, number of cell examined.