Table 3.
Effect of RNAi using different silencer constructs in CNS neurons of Xenopus tadpoles.
| Time | ||||
|---|---|---|---|---|
| Day 3 | Day 5 | |||
| Silencer constructs | G/R ratio (%) | n | G/R ratio (%) | n |
| pSiEnU6-Ctrl | 100.0 ± 9.0 | 137 | 100.0 ± 4.6 | 273 |
| pSiEnU6-shGFP-2 | 80.3 ± 6.6 | 246 | 82.6 ± 5.4 | 340 |
| pSiEnU6-shGFP-3 | 85.7 ± 5.7 | 283 | 86.8 ± 5.6 | 252 |
| pSiEnU6-Ctrl CMV-Ago1 | 100.0 ± 5.9 | 258 | 100.0 ± 6.9 | 150 |
| pSiEnU6-shGFP-2 CMV-Ago1 | 94.8 ± 5.1 | 242 | 93.9 ± 8.5 | 99 |
| pSiEnU6-shGFP-3 CMV-Ago1 | 84.1 ± 8.8 | 167 | 83.0 ± 6.6 | 96 |
| pSiEnU6-Ctrl CMV-Ago2 | 100.0 ± 3.8 | 146 | 100.0 ± 7.0 | 110 |
| pSiEnU6-shGFP-2 CMV-Ago2 | 67.9 ± 4.2 | 138 | 64.0 ± 3.9 | 123 |
| pSiEnU6-shGFP-3 CMV-Ago2 | 66.4 ± 2.1 | 251 | 61.8 ± 3.6 | 123 |
Note: G/R ratio: green to red fluorescence intensity ratio normalized to controls, which contain no shGFP expression in the same pSi configuration. n, number of cells examined.