Figure 3.

Evidence that eotaxin is required for the effects of IL-13. (A, B, and D) Data (mean±sem, n=3) are the numbers of EPO+ cells present by Day 7 in 1 mL liquid cultures established with IL-5 (1 ng/ml), alone or in association with the indicated concentrations of IL-13 (A and D) or eotaxin (B and D) or antibodies (neutralizing antibodies to eotaxin or to CCR3 or control antibody of IgG2a isotype). (C) The amounts of eotaxin detected in 10× concentrated liquid culture supernatants conditioned in the presence of medium alone, IL-5 alone, IL-13 alone, or an association of IL-5 and IL-13. *, P < 0.05, for the differences between the indicated points and the respective IL-5 (negative) controls.