TABLE 1.
Extracellular medium change from 0 mM to 135 mM CI−
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Extracellular medium change from 135 mM to 0 mM Cl−
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Initial influx rate mM/s | Steady-state [Cl−]PL (mM) | Initial efflux rate mM/s | Steady-state [Cl−]PL (mM) | |
Normal controls (n = 5) | 0.31 ± 0.04 | 67.7 ± 7.3 | −0.26 ± 0.05 | 15.9 ± 4.3 |
Normals GlyH-101 (n = 5) | 0.013 ± 0.067b | 27.7 ± 4.4b | −0.095 ± 0.022c | 12.9 ± 4.2 |
CF (n = 5) | 0.043 ± 0.012b | 25.5 ± 3.3b | −0.063 ± 0.13c | 17.0 ± 3.3 |
The influx rate represents the maximal initial slope of the chloride change in mM/s after changing the medium from NaGlu R (0 mM chloride) to NaCl R (135 mM chloride) at the 10-min mark. The efflux rate represents the opposite rate (hence, indicated by negative signs) when the buffer was changed from NaCl R (135 mM chloride) to NaGlu R (0 mM chloride) at the 20-min mark. The steady-state [Cl−]PL represents the phagosomal chloride level 10 min after each respective buffer change.
P < 0.001;
P < 0.05 by Wilcoxon-Mann-Whitney nonparametric analysis (each was compared with the corresponding normal controls). [Cl−]PL, phagosomal chloride concentration.