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. Author manuscript; available in PMC: 2010 Apr 22.
Published in final edited form as: Nat Protoc. 2009 Sep 17;4(10):1471–1501. doi: 10.1038/nprot.2009.98

Table 2. PCR conditions for amplifying Finger 1 pools (steps 41 and 42).

Step number Denature Anneal Extend
1 94°C, 5 min
2-6 94°C, 30 s 55°C, 30 s 72°C, 30 s
7-26 94°C, 30 s 59°C, 30 s 72°C, 30 s
27 72°C, 2 min