| Component | Amount | Final Concentration |
| Plasmid pKJ1712 | 1 μl (1 μg) | 20 ng μl-1 |
| 10× Buffer (NEBuffer 4) | 5 μl | 1× |
| SapI (2 U μl-1) | 5 μl | 0.04 U μl-1 |
| Nuclease-free water | 39 μl | |
| Total | 50 μl | |
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