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. Author manuscript; available in PMC: 2010 Apr 22.
Published in final edited form as: Nat Protoc. 2009 Sep 17;4(10):1471–1501. doi: 10.1038/nprot.2009.98
Component Amount Final Concentration
BbsI-digest from step 38 400 μl
10× BSA (1 mg ml-1) 50 μl 1×
10× BamHI buffer 47.5 μl 1×
BamHI (20 U μl-1) 2.5 μl 0.1 Uμl-1
Total 500 μl