Component	Amount	Final Concentration
Zinc Finger Pool DNA (∼0.15-0.2 μg)	1 μl	3-4 ng μl-1
10× Expand Buffer with MgCl2	5 μl	1×
10mM dNTPs	4 μl	0.8 mM
Forward primer (10 μM)	3 μl	0.6 μM
for F1 use OK1424
for F2 use OK1426
for F3 use OK1428
Reverse primer (10 μM)	3 μl	0.6 μM
for F1 use OK1425
for F2 use OK1427
for F3 use OK1429
Nuclease-free H2O	33.625 μl
Expand Enzyme (3.5 U μl-1)	0.375 μl	0.02625 U μl-1
Total	50 μl