| Step number | Denature | Anneal | Extend |
| 1 | 94°C, 5 min | ||
| 2-11 | 94°C, 30 s | 56°C, 30 s | 72°C, 1 min |
| 12-31 | 94°C, 30 s | 64°C, 30 s | 72°C, 1 min |
| 32 | 72°C, 7 min | ||
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