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. Author manuscript; available in PMC: 2010 Apr 22.
Published in final edited form as: Nat Protoc. 2009 Sep 17;4(10):1471–1501. doi: 10.1038/nprot.2009.98
Component Amount Final Concentration
Purified PCR fragment (from step 52) 10 μl Unknown
10mM dTTP 3 μl 1 mM
10× Cloned Pfu Buffer 3 μl 1×
Cloned Pfu polymerase (2.5 U μl-1) 3 μl 0.25 U μl-1
Nuclease-free water 11 μl
Total 30 μl