| Component | Amount | Final Concentration |
| Purified DNA (from step 54) | 44 μl | Unknown |
| 10× T4 DNA ligase buffer | 5 μl | 1× |
| T4 polynucleotide kinase (10 U ul-1) | 1 μl | 0.2 U μl-1 |
| Total | 50 μl | |
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