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. Author manuscript; available in PMC: 2010 Apr 22.
Published in final edited form as: Nat Protoc. 2009 Sep 17;4(10):1471–1501. doi: 10.1038/nprot.2009.98
Component Amount Final Concentration
pBR-UV5-GP-FD2 backbone 1 μg 20 ng μl-1
PCR fragment encoding three-finger arrays 10 μl Unknown
10× standard T4 DNA Ligase buffer (NEB) 5 μl 1×
T4 DNA ligase (400U μl-1) 2 μl 16 U μl-1
Nuclease-free water to 50 μl