| Component | Amount | Final Concentration |
| pBAC-lacZ plasmid DNA | 1 μg | 50 ng μl-1 |
| 10× NEBuffer 3 | 2 μl | 1× |
| BsaI (10 U μl-1) | 0.5 μl | 0.5 U μl-1 |
| Nuclease-free water | to 20 μl | |
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