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. Author manuscript; available in PMC: 2010 Apr 22.
Published in final edited form as: Nat Protoc. 2009 Sep 17;4(10):1471–1501. doi: 10.1038/nprot.2009.98
Component Amount Final Concentration
SapI-digested pKJ1712 vector backbone 10 μl ∼10 ng μl-1
10× Pfu Buffer 2 μl 1×
dCTP nucleotide (10 mM) 2 μl 1 mM
Cloned Pfu polymerase (2.5 U μl-1) 1.2 μl 1.25 U μl-1
Nuclease-free water 4.8 μl
Total 20 μl