| Component | Amount | Final Concentration |
| Miniprep DNA (of step 98) | 3 μl | ∼15 ng μl-1 |
| 10× NEBuffer 3 | 3 μl | 1× |
| 10× BSA (1 mg ml-1) | 3 μl | 1× |
| Nuclease-free water | 19 μl | |
| PstI (20 U μl-1) | 2 μl | 1.3 Uμl-1 |
| Total | 30 μl | |
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