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. Author manuscript; available in PMC: 2010 Apr 22.
Published in final edited form as: Nat Protoc. 2009 Sep 17;4(10):1471–1501. doi: 10.1038/nprot.2009.98
Component Amount Final Concentration
Binding site oligo #1 (10 μM) 1 μl 50 nM
Binding site oligo #2 (10 μM) 1 μl 50 nM
10× Annealing Buffer 20 μl 1×
Nuclease-free water 178 μl
Total 200 μl