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. Author manuscript; available in PMC: 2010 Apr 22.
Published in final edited form as: Nat Protoc. 2009 Sep 17;4(10):1471–1501. doi: 10.1038/nprot.2009.98
Component Amount Final Concentration
Crude DNA preparation (from step 31) 5 μl Unknown
10× Expand Buffer with MgCl2 5 μl 1×
10 mM dNTPs 4 μl 0.8 Mm
Primer HIS3-2F (10 μM) 1 μl 0.2 μM
Primer HIS3-2R (10 μM) 1 μl 0.2 μM
Expand thermostable polymerase (3.5 U μl-1) 0.375 μl 0.02625 U μl-1
Nuclease-free water 33.625 μl
Total 50 μl