| Component | Amount | Final Concentration |
| pBR-UV5-GP-FD2 plasmid DNA | 40 μg | 100 ng μl-1 |
| 10× NEBuffer 2 | 40 μl | 1× |
| BbsI (5 U μl-1) | 40 μl | 0.5 U μl-1 |
| Nuclease-free water | to 400 μl | |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.