Table 1.
The expression quantification of β-actin and HSPs by the unique Leu-containing isotope peptides in MS
| MOWSE scores(b) (Queries Match) | SILAC-Ratio(e) (mean ± SD; No. of Pep f ) | Unique Leu-containing peptide coverage(%) | ||||||
|---|---|---|---|---|---|---|---|---|
| Protein name | IPI no.(a) | HGTs (c) | PBTs (d) | Ratio1 | Ratio2 | HGTs (c) | PBTs (d) | Change-Ratio(g) (ratio1/ratio2) |
| HSP110/100 family | ||||||||
| HSP H1(105 KD) | IPI00218993 | 77(8) | 140(10) | 0.61 ± 0.02 (2) | 0.82 ± 0 (1) | 2.7 | 1.6 | 0.74 |
| HSP90 family | ||||||||
| HSP90 AB1 | IPI00334775 | 428(18) | 179(10) | 0.29 ± 0.17 (6) | 0.45 ± 0.26 (3) | 8.7 | 4.8 | 0.64 |
| HSP90 AA1 | IPI00382470 | 82(14) | 109(11) | 0.80 ± 0.43 (4) | 0.75 ± 0.52 (2) | 6 | 3 | 1.07 |
| HSP70 family | ||||||||
| HSP A8(71 KD) | IPI00003865 | 486(16) | 360(17) | 0.52 ± 0.09 (8) | 0.55 ± 0.1(3) | 17 | 5 | 0.95 |
| HSP A9(70 KD) | IPI00007765 | 401(16) | 127(5) | 0.49 ± 0.09 (5) | 0.34 ± 0 (1) | 9.6 | 2.2 | 1.44 |
| HSP A5(70 KD) | IPI00003362 | 508 (15) | 99(8) | 0.33 ± 0.15 (7) | 0.57 ± 0.28 (2) | 14 | 3.2 | 0.58 |
| HSP60 family | ||||||||
| HSP D1(60 KD) | IPI00472102 | 67(9) | 419(20) | 0.57 ± 0.08 (3) | 0.52 ± 0.27(4) | 33 | 18 | 1.10 |
| Small HSP family | ||||||||
| HSP B1(27 KD) | IPI00025512 | 159(12) | 138 (10) | 0.29 ± 0.12 (4) | 0.61 ± 0.32 (3) | 8.2 | 7 | 0.48 |
| Control protein | ||||||||
| β-actin | IPI00021439 | 687(31) | 643(39) | 1.03 ± 0.02 (4) | 0.98 ± 0.03 (4) | 12 | 12 | 1.05 |
(a) IPI (International Protein Index) no. is the protein accession number, which was derived from the EMBL-EBI database.
(b) 'MOWSE' is derived from molecular weight search, and it is a method for the identification of proteins from the molecular masses of peptides created by proteolytic digestion and measured by MS.
(c) The protein mixture from HGTs and Leu-d3labeling H4 cells; HGTs: human glioma tissues.
(d) The protein mixture from PBTs and Leu-d3labeling H4 cells; PBTs: para-cancerous brain tissues.
(e) SILAC ratio1 (HGTs/H4) and SILAC ratio2 (PBTs/H4): the peak intensity ratio of Leu-d0-labeling peptides from HGTs or PBTs versus that of Leu-d3-labeling peptides from H4 cells, which was quantified using Analyst QS software.
(f) The number of unique Leu-containing peptides of one protein to calculate SILAC ratio1 and SILAC ratio2.
(g) The Change-Ratio between two tissues (SILAC Ratio1/SILAC Ratio2).