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. 2010 Apr 22;6(4):e1000864. doi: 10.1371/journal.ppat.1000864

Figure 3. Mtb NuoG mediates inhibition of ROS-dependent induction of apoptosis.

Figure 3

(A) THP-1 cells were infected with wild-type (Mtb), ΔnuoG, or complemented mutant (Comp) strains of Mtb or left uninfected (UI). Cultures were incubated in medium alone (−), or in medium containing 15 mM glutathione (GLU) or 10 µM DPI. Apoptotic cells were quantified via TUNEL staining 5 days post infection. (B) Supernatants of the cultures from (A) were harvested on indicated days, and levels of TNF-α were determined by ELISA. (C) Macrophages derived from wild type C57B/6 (B6) or NOX2 deficient gp91 knockout mice (B6 gp91 −/−) were infected with Mtb or MtbΔnuoG. Apoptosis was assayed after 5 days by TUNEL staining. (D) Supernatants of B6 gp91 −/− infected macrophages from experiment shown in (C) were harvested after indicated days and levels of TNF-α were determined by ELISA.