Skip to main content
. 2010 Apr 22;6(4):e1000864. doi: 10.1371/journal.ppat.1000864

Figure 7. Mtb KatG mediates neutralization of phagosomal ROS to inhibit host cell apoptosis.

Figure 7

(A) THP-1 cells were infected with wild type (Mtb) and KatG deficient Mtb (ΔkatG) and assayed for apoptosis via TUNEL staining 3 days post infection. (B) TNF-α concentrations in culture supernatants from (A) were assayed by ELISA. (C) BMDMs from C57B/6 (B6) or gp91 deficient mice (B6 gp91 −/−) were infected with Mtb and ΔkatG, stained with DHE at 24 hrs post infection, and quantified via flow cytometry. (D) Quantification of (C) using Overton cumulative histogram subtraction (FlowJo version 8.8.6 DMV). (E) Fluorescence microscopy of macrophages infected with DiI-labeled bacteria and stained with DCFDA (scale bar  = 10 µm). (C and E) representative samples shown. Arrows indicate phagosomal ROS accumulation.