Skip to main content
. Author manuscript; available in PMC: 2010 Jun 2.
Published in final edited form as: Circulation. 2009 May 18;119(21):2789–2797. doi: 10.1161/CIRCULATIONAHA.108.822403

Figure 4. Activation of calcineurin-NFAT and GATA4 in age-dependent left ventricular hypertrophy and the effect of mCAT.

Figure 4

A) Calcineurin phosphatase activity (nmol phosphate / 5 ug cardiac protein) increased significantly in aged heart and was partially protected in old mCAT hearts. B) Mobility shift assay showed that NFAT DNA-binding activity increased in old WT and the increase was less obvious in old mCAT hearts. Competitive DNA binding with excess unlabelled NFAT probe (arrow) using the leftmost old WT sample confirmed the specificity of the bands (arrowhead). C) The relative mRNA levels of ANP, BNP and MCIP, target genes of NFAT, increased significantly in old WT but not old mCAT hearts. D) Age-dependent increased phosphorylation of GATA-4 at Ser105 was reduced in old mCAT heart. *p<0.025 between YWT and OWT, # p<0.025 between OWT and OmCAT.