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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Plant J. 2010 Jan 6;62(1):92–99. doi: 10.1111/j.1365-313X.2010.04130.x

Figure 2. The rdm12 mutation reduces DNA methylation at RdDM target loci.

Figure 2

(a) Genomic DNA from the indicated genotypes was digested with the DNA methylation sensitive enzyme BstUI, followed by Southern blotting with the RD29A coding sequence as a probe. (b) The genomic DNA was digested with the DNA methylation sensitive enzyme HaeIII, and AtSN1 was amplified. TUB8 was amplified as an internal control. (c) The HaeIII-digested genomic DNA was used for Southern hybridization using the AtMU1 probe. (d) The genomic DNA was digested with HpaII and HaeIII, and followed by Southern hybridization using the 5S rDNA probe.