Skip to main content
. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Plant J. 2010 Jan 6;62(1):92–99. doi: 10.1111/j.1365-313X.2010.04130.x

Figure 4. Identification of the RDM12 gene and mutant complementation assay.

Figure 4

(a) Diagram of the RDM12 genomic sequence. Shown are the positions of exons (solid boxes) and introns, the deletion site in rdm12-1, and the T-DNA insertion site in rdm12-2. (b) Detection of the RDM12 transcript by RT-PCR in the wild type (C24), ros1, ros1rdm12-1, and rdm12-2 and its wild type control (Ws). (c) Genomic DNA from ros1, ros1rdm12-1, wild-type (Ws), and rdm12-2 was digested with the methylation-sensitive enzyme HaeIII and used for amplification of AtSN1. (d) Complementation assay in ros1rdm12-1. The leaves from wild type, ros1, ros1rdm12-1 and six independent RDM12 transgenic T1 lines in the ros1rdm12-1 background were used for luminescence imaging after treatment with 200 mM NaCl for 3 h, and for assaying AtSN1 methylation.