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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: J Cell Biochem. 2010 May;110(1):44–51. doi: 10.1002/jcb.22545

Fig. 2.

Fig. 2

Spatial patterning of EMT correlates with endogenous gradients of cytoskeletal tension. (A) Immunofluorescence staining and (B) frequency map for αSMA expression in rectangular epithelial sheets treated with TGFβ. (C) Sites of EMT correlate with FEM-predicted endogenous mechanical stress. (D) Immunofluorescence staining and (E) frequency map for αSMA expression, and (F) FEM-predicted distribution of mechanical stress in sinusoidal epithelial sheets treated with TGFβ. (G) Frequency of epithelial sheets with patterned αSMA expression after simultaneous treatment with TGFβ and DMSO vehicle, Y27632, ML-7, blebbistatin, or CCG-1423. Scale bars, 50 μm. (**), p<0.005; (***), p<0.0001 compared to DMSO.