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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: J Immunol. 2010 Mar 29;184(9):4725–4732. doi: 10.4049/jimmunol.0903125

Figure 2. Effect of ERAP1 knockdown on S-L presentation from cytosol targeted XXS-L.

Figure 2

HeLa-Kb cells were treated with siRNA targeting ERAP1 or control (ctrl) siRNA then transfected with Ub XXS-L. On day three S-L presentation was determined. FACS showing cells stained with 25.D1.16 following transfection with Ub S-L, Ub GGS-L and Ub WWS-L; cells treated with ctl siRNA (Solid line) or ERAP1 siRNA (dashed line). Shaded histogram, background. Percentage of S-L presentation that is ERAP1 dependent, following HeLa transfected with Ub XXS-L (B). Arrange from highest to lowest, left to right. Calculated from the MFI of cells treated with ctrl (100%) or ERAP1 siRNA. Graphs represent the average percentage of two independent experiments. (C) ERAP1 dependence of Ub XXS-L contructs transfected into MEFs (ERAP1 −/− MEFS compared with ERAP+/+ MEFS) arranged highest to lowest (graphs represent the average percentage of three independent experiments). (D) Scatter graph showing correlation of ERAP1 dependence between MEFs and HeLa cells.