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Immunoblotting for aquaporin 1 (AQP1) in the rat urinary bladder. The anti-AQP antibodies recognize the 27 to 29 kDa bands that correspond to glycosylated AQPs. Anti-GAPDH antibody recognizes the 42 kDa band. The expression of AQP1 protein was significantly increased in the BOO group. The lower panels denote the means±SEs of the 5 experiments for each condition, as determined by the densitometry relative to GAPDH. Con: control, BOO: bladder outlet obstruction, a: p<0.05.
