. Author manuscript; available in PMC: 2010 Apr 23.

Published in final edited form as: Toxicol Appl Pharmacol. 2008 Dec 9;235(2):143–152. doi: 10.1016/j.taap.2008.11.017

Table 4.

Effects of hypersaline conditions on the in vitro biotransformation of fenthion in microsomes of gills from rainbow trout (Oncorhynchus mykiss)

Substrate	Metabolite	Treatment
Substrate	Metabolite	Freshwater	Hypersaline
Fenthion (100 μM)
	Fenoxon	11.7±2.1	12.1±0.2
	S-fenthion sulfoxide	21.5±1.2	11.8±0.8^**
	R-fenthion sulfoxide	18.0±0.5	9.9±0.8^**
	S-fenoxon sulfoxide	bdl	bdl
	R-fenoxon sulfoxide	bdl	bdl
	MMTP (NADPH-independent)	133.5±5.8	127.4±8.1
	MMTP (NADPH-dependent)	117.5±9.5	213.9±40.4^*
Fenoxon (100 μM)
	S-fenoxon sulfoxide	60.4±3.4	29.1±1.4^**
	R-fenoxon sulfoxide	21.7±6.3	9.8±3.3^*
	MMTP (NADPH-independent)	51.6±1.2	68.0±7.4
	MMTP (NADPH-dependent)	123.4±6.1	209.3±11.9^**
S-fenthion sulfoxide (100 μM)
	S-fenoxon sulfoxide	2.1±0.1	bdl
R-fenthion sulfoxide (100 μM)
	R-fenoxon sulfoxide	2.9±0.1	bdl

Data are expressed as pmol/min/mg protein and as mean±SD (n=8 individuals with 3 replicates for each one). Significant differences with respect to freshwater-acclimated rainbow trout indicated by ^* (P<0.05; Student’s t-test) and ^** (P<0.01; Student’s t-test).

MMTP: 3-methyl-4-(methylthio)-phenol.

bdl: below detection limit (<0.3 pmol/min/mg protein).