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. Author manuscript; available in PMC: 2011 Jan 13.
Published in final edited form as: Eur J Neurosci. 2010 Jan 13;31(2):214–222. doi: 10.1111/j.1460-9568.2009.07063.x

Figure 5.

Figure 5

Attenuation of MCM(+)-induced neuronal injury by 4-AP, a Kv channel antagonist. Nuclear morphology and membrane integrity of hippocampal neurons were evaluated by fluorescent dyes PI (red) and DAPI (blue). Panels A-D are fluorescent microscopic images showing addition of MCM(+) to neuronal culture induced neuronal injury (B) and the MCM(+)-induced neuronal injury was significantly attenuated by inclusion of 4-AP in the culture media (C). Survival rates were calculated by counting the number of cells from five different visual fields in each dish containing cultured neurons stained by DAPI and PI dyes are shown in Panel E (n=36 visual fields). Panel F illustrates MCM(+)-associated neuronal injury, assayed using MTT assay (n=36 visual fields). Note that the MCM(+)-induced neuronal injury was blocked by 4-AP. *p=0.002 vs Ctrl, # p=0.01 vs MCM(+), ** p=0.03 vs Ctrl, ## p=0.08 vs MCM(+).