Incubation of MCM(+) with glutamate decarboxylase (GAD) for 3 hr significantly reduced MCM(+)-mediated increase of IA. Panels A-C are current traces recorded from same neuron before (A), during (B) and after (C) bath perfusion of GAD-treated MCM(+)[GAD+MCM+]. The GAD-treated MCM(+) produced a slightly increase of IA, but there is no statistical significance when compared with the recorded during control (Ctrl). However, there is a statistical significance when compared with the IA recorded during bath perfusion of MCM(+), suggesting that glutamate released by MDM plays a partial role in MCM(+)-associated increase of IA. *p=0.223 vs Ctrl, # p≤0.0001 vs Ctrl .