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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Biochim Biophys Acta. 2010 Feb 16;1803(5):591–597. doi: 10.1016/j.bbamcr.2010.02.003

Figure 3. Examination of the interactions between LRBP and UBCE2i in 293T cells.

Figure 3

(A) 293T cells were transiently transfected with 1. pCMV-HA and pCMV-Myc; 2. pCMV-HA-LRBP and pCMV-Myc; 3. pCMV-HA-UBCE2i and pCMV-Myc; 4. pCMY-HA and pCMV-Myc-LRBP; 5. pCMV-HA-LRBP and pCMV-Myc-LRBP; 6. pCMV-HA-UBCE2i and pCMV-Myc-LRBP. 20 mg of whole cell lysate from each transfection were subjected to SDS-PAGE and examined with both Myc and HA antibodies.

(B) The lysates were immunoprecipitated with 10 ml of protein A agarose beads (Myc antibody conjugated) and the samples were subjected to SDS-PAGE and immunoblotted with HA antibody.