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. Author manuscript; available in PMC: 2011 Mar 15.
Published in final edited form as: Clin Cancer Res. 2010 Mar 9;16(6):1834–1844. doi: 10.1158/1078-0432.CCR-09-3123

Figure 2. The iNOS-selective competitive antagonist L-nil blocks iNOS-derived NO production in vitro and in vivo without affecting cell viability.

Figure 2

A. Human melanoma lines mel624, A375, mel526; or colon cancer line WIDR or WIDR transfected with iNOS plasmid were incubated for 72 hours with the indicated concentrations of L-nil, and NO released into the supernatant measured using the nitrite reconversion method. B. The effect of L-nil on NO production by WIDR+iNOS plasmid was used to determine the IC50 of L-nil in the cell culture system. C. Serum was collected 7 hours after intraperitoneal LPS injection of C57BL/6 mice (4 per group) pre-treated for 48 hours with 0.1% L-nil in drinking water (L-nil) or plain drinking water (control) Nitrite levels were determined using the Griess assay. D. Mel624 or A375 melanoma cells were cultured for the indicated length of time in medium containing varying concentrations of L-nil and proliferation was measured by XTT assay.