Skip to main content
. 2010 Apr 23;5(4):e10318. doi: 10.1371/journal.pone.0010318

Figure 1. Celastrol inhibits cell migration.

Figure 1

(A–B) Celastrol inhibits fMLP-induced neutrophil chemotaxis. (A) Freshly isolated human peripheral blood neutrophils were pre-treated with each chemical for 30 minutes, and then subjected to fMLP-mediated chemotaxis for 20 minutes using the EZtaxiscan. The first and the last images of the videos were shown for each drug. fMLP (100 nM), Celastrol (2 µM), 17AAG (4 µM), MG132 (10 µM), and Gedunin (20 µM) were used in the indicated combination. (B) Left panel is the quantification of the migrating speed upon each drug treatment. Right panel is the whole cell lysate assay of the proteasomal activity treated with each drug for 1 hour. The concentration of all chemicals was 5 µM. (C–D) Celastrol reduces epithelial cancer cell motility. (C) The confluent HeLa cells were scratched using a tip. After 10 minutes of recovery, each drug was added and the time-lapse images were taken every 15 minute for 150 minutes. The representative still images of the first (time 0) and the last (time 150) time points were shown. For the sample of “Celastrol-wash”, cells were pre-treated with Celastrol for 3 hour before scratching; and the time-lapse movie was taken in a drug-free medium. (D) Quantification of cell migration. The relative migration area over the control (DMSO-treated) was presented. * indicates p<0.05 by student T-test.