Table 1. Effect of pharmacological agents on mitotic cell death.

Tested chemicals	Targets	# cells examined	# cells dead	% cell death
Vinblastine Only	MT destablizer	98	11	11.2
Celastrol only (4 µM)	Tubulin binding	72	25	34.7
Celastrol (2 µM)		69	27	39.1
Celastrol (4 µM)		61	43	70.5
18-bGCA (20 µM)	Structurally analogous	22	4	18.2
	to Celastrol
17AAG (8 µM)	HSP90	16	2	12.5
MG132 (5 µM)	Proteasome	30	0	0
Etoposide (10 µM)	Topoisomerase	30	6	20
Bay 11-7085 (20 µM)	NF-kappa B	27	1	3.7
Resveratrol (20 µM)	Antioxidant	22	1	4.5
Akt-i VIII (7.6 µM)	Akt	28	5	17.9
LY294002 (20 µM)	PI3K	36	10	27.8

HeLa cells expressing EGFP-Histone H2B (HeLa-H2B) were arrested at mitotic phase by Vinblastine treatment, and the indicated amounts of each drug was added. The apoptotic death of only the ‘pre-arrested’ mitotic cells in 4 hours as identified by time-lapse movie was scored. ‘Vinblastine only’ indicates without addition of other drugs and ‘Celastrol only’ indicates its effect on normal mitotic cells (see Materials and Methods).