Table 1. Effect of pharmacological agents on mitotic cell death.
Tested chemicals | Targets | # cells examined | # cells dead | % cell death |
Vinblastine Only | MT destablizer | 98 | 11 | 11.2 |
Celastrol only (4 µM) | Tubulin binding | 72 | 25 | 34.7 |
Celastrol (2 µM) | 69 | 27 | 39.1 | |
Celastrol (4 µM) | 61 | 43 | 70.5 | |
18-bGCA (20 µM) | Structurally analogous | 22 | 4 | 18.2 |
to Celastrol | ||||
17AAG (8 µM) | HSP90 | 16 | 2 | 12.5 |
MG132 (5 µM) | Proteasome | 30 | 0 | 0 |
Etoposide (10 µM) | Topoisomerase | 30 | 6 | 20 |
Bay 11-7085 (20 µM) | NF-kappa B | 27 | 1 | 3.7 |
Resveratrol (20 µM) | Antioxidant | 22 | 1 | 4.5 |
Akt-i VIII (7.6 µM) | Akt | 28 | 5 | 17.9 |
LY294002 (20 µM) | PI3K | 36 | 10 | 27.8 |
HeLa cells expressing EGFP-Histone H2B (HeLa-H2B) were arrested at mitotic phase by Vinblastine treatment, and the indicated amounts of each drug was added. The apoptotic death of only the ‘pre-arrested’ mitotic cells in 4 hours as identified by time-lapse movie was scored. ‘Vinblastine only’ indicates without addition of other drugs and ‘Celastrol only’ indicates its effect on normal mitotic cells (see Materials and Methods).