Table 1. Primers for specific amplification of Seg-2 of BTV-6, by RT-PCR.

Primer pairs	Individual forward and reverse primers*	Primer Sequence (5′ to 3′)	Position on genome segment 2 (nt)	Predicted Product Size (bp)
Primer pair I	BTV-6/2/301F	GGTGGTATGTATAGAGGAAG	875-894	1631**
	BTV-6/2/790R	ACCACGCTACTCTGTATGCC	2506–2487
Primer pair II	BTV-6/2/153F	CGAGGCGATTGGTGACACAGGT	461-482	2226
	BTV-6/2/853R	CAAAGGGAACCTCGCGCGTAATC	2687–2664
Primer pair III	BTV-6/2/35F	GAGCGAAGATGATGAGGT	107–124	1211
	BTV-6/2/439R	GTCTTCTTCGTCTGTGAGATCAA	1318–1295

*Individual primers are identified by the BTV serotype (e.g. BTV-6) followed by the number 2 (to indicate Seg-2), then a number to indicate the relative amino acid position of the primer within VP2, followed by F or R to indicate forward or reverse orientation.

**This primer pair also amplifies Seg-2 of BTV-14 and BTV-21, the most closely related serotypes to BTV-6 within nucleotype ‘C’ and it is therefore regarded as nucleotype ‘C’ specific.