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. 2010 Apr 23;5(4):e10325. doi: 10.1371/journal.pone.0010325

Figure 4. Signaling complexes in WT and AKAP5 mutant hippocampus.

Figure 4

A, Immunoprecipitation (IP) of AKAP5, calcineurin (CaN), and RIIα, with immunoblotting (IB) using antibodies against CaN, AKAP5, and RIIα. Input is the equivalent amount of hippocampal extract. B, IP of the dendritic shaft AKAP MAP2, AKAP5, a PSD enriched protein, GluR2/3, and RIIα, with immunoblotting (IB) using antibodies against AKAP5, PSD-95, RIIα, MAP2, and GluR2/3. IP's are 7.5 fold more concentrated relative to the inputs, to facilitate visualization and comparison of the WT vs. KO extracts, as well as to detect weaker interactions.

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