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. 2010 Apr 23;5(4):e10341. doi: 10.1371/journal.pone.0010341

Figure 1. Identification of WRN acetylation sites.

Figure 1

A, acetylation assay in cells with different acetyltransferases. Western blot analysis was performed with anti-acetylated lysine antibody (Upper panel) or with anti-WRN antibody. The experiment was repeated at least two times. B, mass-spectrometry analyses identify the WRN acetylation sites. Acetylated WRN protein was purified by co-transfection of FLAG-tagged WRN with both CBP and p300. The protein was digested with trypsin and analyzed by both MALDI-TOF and LC-ESI MS/MS. Scores of ESI or MALDI QIT were shown. Mascot scores greater than 40 or found in both MALDI and ESI were most confident for the true detection of acetylation.