Figure 2. Confirmation of WRN acetylation sites in cells using acetylation assay.

A, WRN-WT or single mutant was transfected with CBP into HEK293 cells. After immunoprecipitation with FLAG M2 beads, the IP products were subjected to SDS-PAGE and Western blotting with anti-acetylated lysine and anti-WRN antibodies. B, WRN-WT or double mutant or triple mutant or quadruple mutant or 6KR mutant was transfected with CBP into cells. The same assay described in A was performed. All the experiments were repeated at least three times. The bands of WRN proteins were quantified by ImageJ software.