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. 2010 Apr 23;5(4):e10336. doi: 10.1371/journal.pone.0010336

Figure 1. Dynamics of centromere coupling.

Figure 1

Meiotic cells (DDO45 and DDO46) were evaluated for the behavior of centromeres by indirect immunofluorescence observation of kinetochores (Mtw1-13XMYC), a pair of homologous centromeres (GFP-tagged CEN1) and Zip1, in chromosome spreads. (A) The number of Mtw1-13XMYC foci was determined at each time point (n>100 for each time point). C: clustered (<12 Mtw1 foci); P: paired (either between homologs or non homologs: 12–20 Mtw1 foci); D: dispersed (>20 Mtw1 foci) (B) Examples of categories of centromere organization: clustered, dispersed, non-homologous coupled (12–20 Mtw1 foci, separate CEN1-GFP foci), and homologous paired (12–20 Mtw1 foci, one CEN1-GFP focus). Mtw1: red; CEN1: yellow; Zip1: green. Scale bar: 2µm (C) The proportion of chromosome spreads in each category (DDO45 and DDO46; n>50 for each time point). Three iterations of this experiment can be seen. The percentages of cells with punctate and linear Zip1 staining are shown as a reference of meiotic progression in each individual experiment.