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. Author manuscript; available in PMC: 2010 Apr 23.
Published in final edited form as: Biochimie. 2008 Oct 7;91(2):300–303. doi: 10.1016/j.biochi.2008.09.002

Fig. 2.

Fig. 2

Rgt1 is efficiently recruited by artificially multimerized binding sites without intervening sequences and mediate synergistic repression of transcription.

A. Electromobility shift assay with the 32P-labelled oligonucleotides containing different numbers of Rgt1 binding sites (1x, 2x, 3x, 4x, and 6x) and the different amounts of N-terminal fragment of Rgt1 (0, 2.5, 5, 10, 20, 40, 80, 120, 250, and 500 ng per lane, from the left).

B. The complementary oligonucleotides containing different numbers of the Rgt1 binding sites were inserted between the LEU2 upstream activation sequence and the TATA box of HIS3 fused to lacZ [4]. β-Galactosidase activity was assayed as described in Fig. 1.

C. Different HXT promoters have different numbers of the Rgt1 binding sites. Black circles indicate positions of the Rgt1 consensus binding site sequence (5′CGGANNA3′).