Figure 4. The C-terminal residues of Blm10 are important for its function.

(A) Isogenic strains were constructed in the A364a genetic background with the genotypes indicated (Table S1). Multiple independent colonies of each strain growing on glycerol medium to select for retention of mitochondrial function were used to inoculate rich medium containing glucose. Saturated cultures were diluted and plated on rich glucose medium, then mitochondrial function in clones was assessed using the tetrazolium staining method (Ogur et al., 1957). Results from multiple strains with the same genotype were combined (Table S1, total number indicated as N), with the average percentage yield of petite colonies plotted here. Error bars indicate the standard deviation of the measurements.

(B) As in panel A, except isogenic pairs from three other commonly used genetic backgrounds containing or lacking Blm10 were assayed by picking 120 colonies without regard to size, then replica plating to media containing glycerol or glucose to determine the number of petite colonies (Table S1). See also Figures S3.