Table 1.
Proteasome:Blm10 crystallographic data statistics.
| Crystallographic Data | |||||||
|---|---|---|---|---|---|---|---|
| ID/namea | c158/FL-1 | c164/FL-thim | c172/FL-PtCl4 | c280/NΔ50-1 | c290/NΔ50-MeHg | c292/NΔ50-PtCl4 | |
| Spacegroup | P212121 | P21 | P212121 | P21 | P21 | P21 | |
| Cell | a (Å) | 124.1 | 238.2 | 128 | 236.1 | 238.7 | 237.8 |
| b (Å) | 238.5 | 126.2 | 236.2 | 127.8 | 127.8 | 128.6 | |
| c (Å) | 489.8 | 528.7 | 515.1 | 532.3 | 535.6 | 537.1 | |
| β (°) | 102.5 | 102.8 | 102.5 | 102.8 | |||
| Resolution (Å)b | 50–3.9 (4.0–3.9) | 40–6.2 (6.4–6.2) | 50–6.0 (6.2–6.0) | 30–3.4 (3.5–3.4) | 30–6.8 (7.0–6.8) | 30–6.8 (7.0–6.8) | |
| Rmerge (%) | 20.9 (42.8) | 11.0 (43.6) | 18.2 (58.3) | 10.3 (31.4) | 9.6 (36.5) | 9.4 (35.9) | |
| I/σ(I) | 6.3 (2.2) | 8.6 (2.1) | 9.3 (2.4) | 10.3 (2.4) | 9.7 (2.0) | 9.4 (2.1) | |
| Completeness | 98.2 (97.3) | 99.6 (99.9) | 99.2 (97.7) | 98.6 (88.6) | 99.8 (99.6) | 99.8 (99.9) | |
| Refinement Statistics | |||||||
|---|---|---|---|---|---|---|---|
| Resolution (Å)b | 30–3.0 | ||||||
| # reflns used in refinementc | 490,961 | ||||||
| Rwork/Rfree (%) | 19.6/25.0 | ||||||
| Number of atomsc | 158,904 | ||||||
| <B> (Å2) | 104.3 | ||||||
| RMSD bond lengths (Å)d | 0.01 | ||||||
| RMSD bond Angles (°) | 1.291 | ||||||
Values in parentheses refer to the high-resolution shell.
Each data set was collected from a single crystal, which was given a unique identifier and a more descriptive name. Crystals were of full-length (FL) Blm10 or Blm10 missing the first 50 amino acid residues (Δ50). Mercury (Hg) or Platinum (Pt) heavy atom derivatives were prepared as described in Supplementary Experimental Procedures.
Resolution of a data set was formally defined as the Bragg spacing at which half of the measured reflections have an I/σ(I) value of at least 2.0, although the data were processed and used to smaller Bragg spacing. All crystallographic data values in this table refer to reflections within the formal resolution limits, whereas the refinement statistics refer to all data.
The total number of all 20S and Blm10 non-Hydrogen atoms in the asymmetric unit. No solvent molecules were included in the model.
RMSD denotes root mean square deviation from ideality.