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. Author manuscript; available in PMC: 2010 Apr 23.
Published in final edited form as: J Cell Sci. 2008 Jul 15;121(Pt 15):2601–2611. doi: 10.1242/jcs.016089

Fig. 1. Kalirin7 produces a Cdk5-dependent response in PC12 cells.

Fig. 1

A. Domains of Kalirin7 and its N-terminal Δ-splice variant are shown; S, Sec14P- like; DH, Dbl homology; P, pleckstrin homology; T1590PAK, putative Cdk5 phosphorylation site. B. PC12 cells were fixed 24h after transfection with vectors encoding GFP or Kalirin7. Transfected GFP protein was visualized using fluorescence or Myc antibody (green); filamentous actin was visualized using TRITC-phalloidin (red). White arrowheads, protrusions; stars, non-transfected cells; arrow, nucleus. Scale, 10μm. C. PC12 cells transfected with dual promoter vector encoding GFP (green) and DN-Cdk5 (blue); TRITC-phalloidin (red). GFP-expressing cells always expressed DN-Cdk5. D. PC12 cells co-transfected with vectors encoding Kalirin7 (myc; blue) and GFP/DN-Cdk5 (GFP, green); TRITC-phalloidin (red). White stars, non-transfected; scale, 10μm. E. Using the GFP or myc signal to identify cell margins, perimeter and cell roundness were evaluated using SimplePCI software: control (GFP), n=18; Kalirin7 (Kal7), n=15; Kalirin7 with DN-Cdk5, n=24; p-values calculated using the Student’s t-test (two-tailed) and standard error of the mean plotted; #, p<0.001.