Skip to main content
. Author manuscript; available in PMC: 2010 Apr 23.
Published in final edited form as: J Cell Sci. 2008 Jul 15;121(Pt 15):2601–2611. doi: 10.1242/jcs.016089

Fig. 4. The GEF activity and spectrin repeat region of Kalirin7 are required for protrusion formation.

Fig. 4

A. PC12 cells transfected 24h earlier with vector encoding inactive Kalirin7-ND/AA were fixed; Kalirin7 was visualized using myc antibody (green); filamentous actin visualized using TRITC-phalloidin (red). No morphological differences were detected in cells expressing Kalirin7-ND/AA. B. PC12 cells expressing GFP fusion proteins of constitutively active (CA)-Rac or CA-RhoG were fixed after 24h. Fusion proteins visualized based on GFP fluorescence (green); filamentous actin visualized with TRITC-phalloidin (red); white stars, non-transfected cells. C. PC12 cells expressing KGEF1→7end or the T/A and T/D mutants were visualized with myc antibody (blue), filamentous actin was visualized simultaneously. Scale: 10μm.