Skip to main content
. Author manuscript; available in PMC: 2010 Apr 23.
Published in final edited form as: J Cell Sci. 2008 Jul 15;121(Pt 15):2601–2611. doi: 10.1242/jcs.016089

Fig. 8. GEF activity of Kalirin is increased by mutation of Thr1590 to Asp.

Fig. 8

GEF activity was assessed in vitro using GDP-Mant-loaded Rac1 (2μM). A. Change in fluorescence with time is shown; buffer or the proteins indicated (final concentration 20μM) were added at t=3min. B. Data from the first 20min converted to a semi-logarithmic plot enabled initial rate calculation. C. Data from three assays for GEF activities of GST-KGEF1→K7end, KGEF1→K7endT/A and KGEF1→K7endT/D averaged. Significant differences indicated; *, p<0.05.