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. Author manuscript; available in PMC: 2010 Apr 23.
Published in final edited form as: J Cell Sci. 2008 Jul 15;121(Pt 15):2601–2611. doi: 10.1242/jcs.016089

Fig. 9. Solubility of Kalirin7-T/D is increased.

Fig. 9

A. Cortex from adult rat brain was separated into cytosol, organelle and TX-100 insoluble fractions; 20μg of protein from each fraction was subjected to Western blot analysis using antibody specific for Kalirin7. B. PC12 cells expressing wildtype Kalirin7, Kalirin7-T/A and Kalirin7-T/D were separated into soluble and insoluble fractions as described in Methods; 10% of the soluble fraction (Sol) and 1% of the resuspended pellet fraction (Pel) was subjected to Western blot analysis for myc. Bar graph: quantification of data from two independent experiments; error bar gives range.