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Iron-induced amide chemical shift perturbations at varying iron:frataxin concentration ratios. Frataxin secondary structure information is given at the top. Perturbations in backbone 1H and 15N resonances, induced by the addition of Fe(NH4)2(SO4)2 to 15N-labeled apofrataxin, at the following iron:protein stoichiometries are plotted: (A) 2:1, (B) 1.5:1, (C) 1:1, and (D) 0.5:1.
