Table 1.
LtxA induces cell death in various human hematologic malignant cell lines.
| Disease/Cell line | IC50 value | FDC1 | CD11a2 | CD182 |
|---|---|---|---|---|
| Acute myeloid leukemia | ||||
| HL-60 | 200 ng/ml | >99 | 90 | 90 |
| THP-1 | 8 ng/ml | >99 | 96 | 95 |
| GDM-1 | 200 ng/ml | 98 | ND3 | ND |
| CMK | >10 μg/ml | ND | ND | ND |
| Acute lymphoblastic leukemia | ||||
| Jurkat | 200 ng/ml | >99 | ND | ND |
| Loucy | 300 ng/ml | 67 | 32 | 89 |
| Molt-4 | 30 ng/ml | 93 | ND | ND |
| Chronic myelogenous leukemia | ||||
| KU812 | 300 ng/ml | 96 | ND | ND |
| K562 | >10 μg/ml | ND | <1 | <1 |
| MEG-01 | >10 μg/ml | ND | ND | ND |
| Non-Hodgkin's lymphoma | ||||
| RL | 50 ng/ml | >99 | ND | ND |
| Toledo | 5 ng/ml | 81 | 85 | 86 |
| Burkitt's lymphoma | ||||
| Daudi | ND | 25 | 17 | 20 |
| CA46 | ND | 29 | 2 | 2 |
| Histiocytic lymphoma | ||||
| U937 | 80 ng/ml | 97 | ND | ND |
| Multiple myeloma | ||||
| RPMI 8226 | ND | 34 | ND | ND |
| Non-WBC lines | ||||
| Hep G2 (liver) | >10 μg/ml | ND | ND | ND |
| MCF7 (mammary) | >10 μg/ml | ND | ND | ND |
| A549 (lung) | >10 μg/ml | ND | ND | ND |
| ECV304 (bladder) | >10 μg/ml | ND | ND | ND |
| HGF-1 (gingiva) | >10 μg/ml | ND | ND | ND |
1
FDC-Fraction of dead cells after 24-treatment with 2.0 μg/ml LtxA, percent dead
2
Percent positive
3
ND - Not determined