Table 1. Putative PKA substrates and interacting proteins in S. mansoni and S. japonicum.
Putative S. mansoni and S. japonicum homologues of known PKA substrates and interacting proteins were identified by BLAST analysis. GenPept names and accession numbers for all proteins are provided. Full GenPept descriptors of the proteins are as follows: SLC4A4, solute carrier family 4, sodium bicarbonate cotransporter, member 4 isoform 1; HDAC-8, histone deacetylase 8; PPP1R9B, protein phosphatase 1, regulatory subunit 9B; kin-2, type II PKA regulatory subunit; CNR_1, cannabinoid receptor 1 isoform a; Bub2p, protein containing a TBC (Tre-2/Bub2/Cdc16) domain; nbc2 3, sodium bicarbonate cotransporter; SmHDAC1, histone deacetylase; Sm06231, hypothetical protein; Smp_030400, type I-beta PKA regulatory subunit; Smp_043260, histamine-responsive G protein coupled-receptor; Smp_001420; Gh regulated tbc protein-1; HDAC1/2, histone deacetylase 1/2; SJCHGC09512, PDZ (post synaptic density) domain-containing protein; SJCHGC07612, hypothetical protein; SJCHGC08537, hypothetical protein.
| PKA substrate/interacting protein | Putative schistosome homologue | PKA phosphorylation sites in schistosome homologue |
|---|---|---|
| S. mansoni | ||
| Homo sapiens SLC4A4 (NP 001091954) | nbc2 3 (XP 002572546) | S253, T447, S1230 |
| H. sapiens HDAC-8 (AAH50433) | SmHDAC1 (XP 002571987) | S359 |
| H. sapiens PPP1R9B (NP 115984) | Sm06231 (XP 002575145) | S41, S766 |
| Caenorhabditis elegans kin-2 (NP 001076771) | Smp_030400 (XP 002574488) | S191 |
| H. sapiens CNR_1 (NP 057167) | Smp_043260 (XP 002575668) | T65 |
| Saccharomyces cerevisiae Bub2p (NP 013771) | Smp_001420 (XP 002571615) | S41 |
| S. japonicum | ||
| H. sapiens HDAC-8 (AAH50433) | HDAC1/2 CAX72896 | S407 |
| H. sapiens PPP1R9B (NP 115984) | SJCHGC09512 AAX26695 | S273 |
| H. sapiens CNR_1 (NP 057167) | SJCHGC07612 AAX28307 | T78 |
| Saccharomyces cerevisiae Bub2p (NP 013771) | SJCHGC08537 AAW24955 | S41 |